IGF::CL::IGF PROJECT TITLE: "AN SSR-BASED APPROACH FOR PRIORITIZING REGENERATION OF CUCURBITA MSCHATA." PROJECT WORK INCLUDES: FOUR DNA SAMPLES OF CUCURBITA MOSCHATA WILL BE PRESCREENED FOR POLYMORPHISMS USING 20 PCR PRIMER PAIRS (OLIGOS/PRIMERS) PROVIDED BY THE CONTRACTOR. BASED ON THE RESULTS OF THIS INITIAL PRESCREENING, THE 12 PRIMER PAIRS THAT ARE IDENTIFIED AS MOST EFFICACIOUS IN DETECTING POLYMORPHISMS WILL THEN BE USED TO SCREEN ~200 DNA SAMPLES OF CUCURBITA MOSCHATA FOR SSR FRAGMENT LENGTH POLYMORPHISMS. ON COMPLETION OF THE ANALYSIS, THE CONTRACTOR WILL PROVIDE COPIES ALL DATA COLLECTED ON ALL SAMPLES INCLUDING FRAGMENT SIZES (IN BP) OF AMPLIFIED PCR PRODUCTS CORRESPONDING TO THE SSR LOCI. (CUCURBIT CURATOR: DR. ROBERT JARRET)